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alphaA-Conotoxin PIVA

alphaA-Conotoxin PIVA

Item no.	ALO-STC-600-1mg
Manufacturer	Alomone
Amount	1 mg
Quantity options	0.1 mg 0.5 mg 1 mg 50 ug 5 mg
Category	Neuroscience Molecular Targets for Neuroscience Reagents & Chemicals Molecules
Type	Molecules
Format	Lyophilized
Specific against	other
Purity	≥98% (HPLC)
Formula	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Sequence	GCCGSYXNAACHXCSCKDRXSYCGQ-NH<sub>2</sub>
ECLASS 10.1	32169090
ECLASS 11.0	32169090
UNSPSC	12000000
Shipping Condition	Room temperature
Available
Manufacturer - Type	Non Antibodies
Manufacturer - Applications	Electrophysiology
Manufacturer - Category	Proteins
Manufacturer - Targets	α1/β1/γ/δ nAChR
Country of Origin	Israel
Shipping Temperature	The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Storage Conditions	Storage as Solution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Molecular Weight	2649 Da
Manufacturer - Format	Lyophilized
Short description	A Potent Antagonist of Muscle Fetal α1/β1/γ/δ nAChR
Description	Alpha-A-conotoxin PIVA, Alpha-conotoxin PIVA - A Potent Antagonist of Muscle Fetal α1/β1/γ/δ nAChR
Reconstitution	Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Specificity	A Potent Antagonist of Muscle Fetal α1/β1/γ/δ nAChR
PH	7, 4
UNSPSC	12352202
Origin	Conus purpurascens (Purple cone)
Modifications	Disulfide bonds between: Cys²-Cys¹⁶, Cys³-Cys¹¹and Cys¹⁴-Cys²³ X = Hydroxyproline Gln²⁵ - C-terminal amidation
Effective Concentration	1 - 20 nM
Activity	αA-Conotoxin PIVA reversibly blocks muscle α1/β1/γ/δ nicotinic ACh receptor1, 2.
Solubility	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Sterile endotoxin free	no
Bioassay tested	yes
Scientific Background	αA-Conotoxin PIVA is the first member of a new family of nAChR-targeted Conus peptides, named short αA-conotoxins1. It is a peptide toxin originally isolated from Conus Purpurascens (Purple cone) venom1.This peptide reversibly blocks postsynaptic muscle fetal α1/β1/γ/δ nicotinic ACh receptor1, 2 . It also blocks postsynaptic muscle adult α1/β1/ε/δ nicotinic ACh receptor, with lower affinity (IC50 of 22 nM to the adult compared to 2.3 nM to the fetal nAChR)2.αA-conotoxin PIVA has a strikingly different amino acid sequence and a Cys framework from all other nAChR-targeted peptides previously characterized from Conus venoms. Despite the striking structural divergence, the peptide blocks the ACh binding site of the nAChR at the neuromuscular junction, similar to the well known typical of a-conotoxins1.The characterization of the short αA-conotoxins revealed diverse kinetics of a block of the fetal muscle nAChR, particularly in dissociation rates. The structure-function relationships of native αA-conotoxins revealed a single amino acid locus (alternatively either His or Pro) that is a critical determinant of the dissociation kinetics2.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ALO-STC-600-1mg-safety-datasheet.pdf