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alpha-Conotoxin EI

alpha-Conotoxin EI

Item no.	ALO-STC-900-5mg
Manufacturer	Alomone
CASRN	170663-33-9
Amount	5 mg
Quantity options	0.1 mg 0.5 mg 10 mg 1 mg 5 mg
Category	Food Safety Allergenes Neuroscience Molecular Targets for Neuroscience Reagents & Chemicals Molecules
Type	Molecules
Format	Lyophilized
Specific against	other
Purity	≥98% (HPLC)
Formula	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Sequence	RDXCCYHPTCNMSNPQIC-NH<sub>2</sub>
ECLASS 10.1	32169090
ECLASS 11.0	32169090
UNSPSC	12000000
Shipping Condition	Room temperature
Available
Manufacturer - Type	Non Antibodies
Manufacturer - Applications	Electrophysiology
Manufacturer - Category	Proteins
Manufacturer - Targets	α1/β1/γ/δ nAChR
Country of Origin	Israel
Shipping Temperature	The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Storage Conditions	Storage as Solution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Molecular Weight	2093.4 Da
Manufacturer - Format	Lyophilized
Short description	An Antagonist of α1/β1/γ/δ nAChR
Description	Alpha-conotoxin EI - An Antagonist of α1/β1/γ/δ nAChR
Reconstitution	Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Specificity	An Antagonist of α1/β1/γ/δ nAChR
PH	7, 4
UNSPSC	12352202
Origin	Conus ermineus (Agate cone) (Chelyconus ermineus)
Modifications	Disulfide bonds between: Cys⁴-Cys¹⁰, Cys⁵-Cys¹⁸ Cys¹⁸ - C-terminal amidation X = Hydroxyproline
Effective Concentration	500 nM - 1 µM
Activity	A selective blocker of α1/β1/γ/δ nAChR.
Solubility	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Sterile endotoxin free	no
Bioassay tested	yes
Scientific Background	The nicotinic acetylcholine receptors (nAChRs) are a family of ligand-gated ion channels that are widely expressed throughout the central and peripheral nervous systems. The best characterized nAChR is the receptor at the neuromuscular junction, with four different subunits in a pentameric array, (α1)2β1γδ1.Small peptide toxins of Conus origin known as the Aα-conotoxins are highly useful tools for exploring ligand nAChR interactions. α-Conotoxins are known to be selective and potent competitive antagonists of nicotinic acetylcholine receptors2.α-Conotoxin EI is a 18 amino acid peptidyl toxin, originally isolated from the Conus ermineus (Athlantic fish-hunting cone) venoM In Torpedo nAChRs, α-conotoxin EI selectively binds the agonist site near the α/δ subunit interface. In mammalian nAChRs α-conotoxin EI shows high affinity for both the α/δ and α/γ subunit interfaces (with some preference for the α/δ site)3.The unique binding preference of α-conotoxin EI to the α/δ subunit interface makes it a valuable structural template for superposition of various α-conotoxin possessing distinct receptor subtype specificities4.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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ALO-STC-900-5mg-safety-datasheet.pdf