HBC514

>98%

C21H22N4

No Development Reported

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells.
IC50 & Target: IC50: RNA fluorescent dye
In Vitro: HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. 'Pepper' is able to enhance HBC's fluorescence more than 3, 000-fold and exhibits bright fluorescence when expressed in living cells.
'Pepper' has reduced Mg2+ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness.
HBC514 (2-10 uM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9).