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Monkeypox Virus Protein Array G1

Monkeypox Virus Protein Array G1

Item no.	PAV-MPHM-G1-16
Manufacturer	Raybiotech
Amount	1 slide (16 wells)
Quantity options	1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells) 1 slide (16 wells) 2 slides (32 wells)
Category	Arrays Protein Arrays
Type	Array
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rabbit (Oryctolagus cuniculus)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Manufacturer - Category	Arrays\|Disease Focused Arrays\|Protein Arrays
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Compatible Sample Types	Ascites Hybridoma cell culture medium Other fluids Plasma Purified antibodies Purified protein Serum
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Kit Components	Ray Bio® monkeypox virus protein array Blocking Buffer Wash Buffer I Wash Buffer IIBiotin-conjugated secondary antibody Cy3 equivalent dye conjugated streptavidin Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)
Other Materials Required	Distilled water Small plastic boxes or containers Orbital shaker or oscillating rocker Pipettors, pipette tips and other common lab consumables Aluminum foil Laser fluorescence scanner View Compatible Laser Scanners Don't have a compatible scanner? Ray Biotech now offers F REE scanning service for all RayBio glass slide antibody arrays! Learn More
Product Features	Small sample volume required High density High sensitivity Large dynamic range Suitable for high-throughput assays
UNSPSC Code	41116133
Protein Name & Synonyms	A29L, A30L, A33R, A35R, B16R, C19L (identical to vaccinia virus F13L), D6L, E8L, H3L, I1L, L1R
Introduction	Recombinant Monkeypox virus (MPXV) proteins derived from bacterial and human cells (HEK293) are spotted in triplicate on a standard glass slide (25 mm x 75 mm x 1 mm), along with positive and negative controls to monitor each incubation step. When liquid sample types, such as serum or purified antibodies, are incubated on the array, "primary" antibodies in the sample will bind to their specific target proteins. Specific primary antibody isotypes (IgG, IgM, or IgA) from serum, plasma, purified antibodies, etc. are then targeted by biotin-conjugated anti-IgG, IgM, or IgA secondary antibodies, respectively, that are specific for the host species. A fluorophore-conjugated streptavidin molecule is then added, which binds to the biotin on the secondary antibody. The fluorophore enables the detection of the immobilized primary antibody via fluorescence using a laser scanner. The fluorescence signal is proportional to the amount of immobilized antibody. Since each spot represents a unique protein that is known, the specific target(s) bound by the antibodies can be ascertained.
Application Notes	Vaccine Development: Measure IgG, IgM, or IgA antibody responses to immunization or patients Protein-protein Interaction Studies: Determine the interactions between the viral proteins and other molecules, such as proteins, peptides, and small molecules. (Additional reagents and materials not included in the kit may be required.)
Number of Proteins Detected	11.0

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.