Ala5-Galanin (2-11)

Aequorin functional assay / Calcium imaging assay

Galanin 2 receptors

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

1120 Da

A Potent and Selective Agonist of Galanin 2 Receptor

Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. Avoid multiple freeze-thaw cycles to maintain biological activity. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%–0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.

7, 4

Leu¹⁰ - C-terminal amidation

Ala5-Galanin (2-11) is a Galanin 2 receptor agonist1.

no

Ala5-Galanin (2-11) is a galanin analogue that acts as a potent, selective and specific Galanin 2 receptor (GalR2) agonist. Ala5-Galanin (2-11) is a full agonist of Gal2R with no detectable binding to human galanin receptors 1 and 3. Ala5-Galanin (2-11) is based on the short galanin fragment galanin (2-11) including a single amino acid substitution of alanine instead of serine at position five.To date, Ala5-Galanin (2-11) is the shortest galanin receptor 2 specific agonist reported1. Ala5-Galanin (2-11) activates GalR2 with an EC50 value of 1.01 ± 0.18 μM1.Galanin receptors belong to the family of G-protein coupled receptors and consist of three subtypes: GalR1, GalR2, and GalR32.