Comparison

Human CCR8 Protein (Nanodisc, Flag-Strep & His Tag) European Partner

Item no. 11450-H91H-NA-100
Manufacturer Sino Biological
Amount 100 ug
Quantity options 100 ug 20 ug 500 ug
Category
Type Proteins Recombinant
Specific against Human (Homo sapiens)
Host HEK293 cells
Conjugate/Tag Strep, FLAG, HIS
Purity ≥ 85 % as determined by SDS-PAGE.
Dry ice Yes
Sequence Met1-Cys228 & Asn230-Ser318
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CC-CKR-8,CCR-8,CDw198,CKR-L1,CKRL1,CMKBR8,CMKBRL2,CY6,GPR-CY6,GPRCY6,TER1
Shipping Condition Dry ice
Available
Manufacturer - Type
Protein
Manufacturer - Conjugate / Tag
N-Flag-Strep, C-His
Shipping Temperature
Dry ice
Storage Conditions
-20C
Molecular Weight
The recombinant Human CCR8 protein predicts a molecular mass of 46.51 kDa. The recombinant protein migrates as an approximately 96.9 kDa (dimer) and 44 kDa protein in SDS-PAGE under reducing conditions.
Antigen molecule name
CCR8
Buffer
Supplied as sterile 50 mM HEPES, 150 mM NaCl, pH 7.5.
Protein Construction
A DNA sequence encoding the Human CCR8 (P51685)(Met1-Cys228) & (Asn230-Ser318) was expressed and linked by a Fusion Protein, with a Flag tag followed by a Strep Tag at the N-terminus, a polyhistidine tag at the C-terminus. Within the expressed regions, there are several proprietary thermostabilizing mutations.This protein was prepared using our Nanodisc platform. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Bio-Activity
1. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind BMS-986340-Derived Anti-Human CCR8 Monoclonal Antibody, Human IgG1, the EC50 is 2-10 ng/mL (QC tested).
2. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind S-531011-Derived Anti-Human CCR8 Monoclonal Antibody, Human IgG1, the EC50 is 3-10 ng/mL (Routinely tested).
3. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind JTX-1811-Derived Anti-Human CCR8 Monoclonal Antibody, Human IgG1, the EC50 is 3-10 ng/mL (Routinely tested).
4.Loaded BMS-986340-Derived Anti-CCR8 Antibody, Human IgG1 on proA Biosensor, can bind Recombinant Human CCR8 Protein, Nanodisc, Flag-Strep & His tag (Cat. No. 11450-H91H-NA) with an affinity constant of 2.380 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
5.Loaded S-531011-Derived Anti-CCR8 Antibody, Human IgG1 on proA Biosensor, can bind Recombinant Human CCR8 Protein, Nanodisc, Flag-Strep & His tag (Cat. No. 11450-H91H-NA) with an affinity constant of 1.550 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
6.Loaded JTX-1811-Derived Anti-CCR8 Antibody, Human IgG1 on proA Biosensor, can bind Recombinant Human CCR8 Protein, Nanodisc, Flag-Strep & His tag (Cat. No. 11450-H91H-NA) with an affinity constant of 2.460 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
N-terminus
Met 1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
available

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