[Novoprotein] Recombinant Human Interleukin-27/IL-27 (C-6His)

Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Recombinant Human Interleukin-27 is produced by our Mammalian expression system & the target gene encoding Arg21-Lys229&Phe29-Pro243 is expressed with a 6His tag at the C-terminus.

IL-27 is a heterodimeric cytokine which belongs to the IL-6/IL-12 family of long type I cytokines. It is expressed on monocytes, endothelial cells & dendritic cells. IL-27 is an early product of activated antigen-presenting cells & drives rapid clonal expansion of naive CD4(+) T cells & plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 potentiates the early phase of TH1 response & suppresses TH2 & TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- & ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 & STAT5 phosphorylation & activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. IL-27 has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy. IL-27 reveals to be a potent inhibitor of TH17 cell development & of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 & CD8 T-cells. IL-27 has also an effect on cytokine production. It suppresses proinflammatory cytokine production such as IL2, IL4, IL5 & IL6 & activates suppressors of cytokine signaling such as SOCS1 & SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 & MIG/CXCL9.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.