NDUFS3, Recombinant, Human, aa37-264, His-Tag (NADH Dehydrogenase [Ubiquinone] Iron-sulfur Protein 3, CI-30)

Blue Ice

28, 7

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, also known as NDUFS3, belongs to the complex I 30kD subunit family. The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits.

Source:
Recombinant protein corresponding to aa37-264 from human NDUFS3 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~28.7kD (249aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MESAGADTRP TVRPRNDVAH KQLSAFGEYV AEILPKYVQQ VQVSCFNELE VCIHPDGVIP VLTFLRDHTN AQFKSLVDLT AVDVPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPIES AVSVFKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG HPFRKDFPLS GYVELRYDDE VKRVVAEPVE LAQEFRKFDL NSPWEAFPVY RQPPESLKLE AGDKKPDAK

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.