RNASE3, Recombinant, Human, aa28-160, His-Tag (Eosinophil Cationic Protein, ECP, RNS3, Ribonuclease 3)

Blue Ice

19, 9

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.

RNASE3 is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. It possesses a wide variety of biological activities. This protein exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains and promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content

Recombinant protein corresponding to aa28-160 from human RNASE3 at N-terminal, fused to His-tag expressed in E. coli. Accession: AAH96060

Molecular Weight:
~19.9kD (171aa)

Amino Acid Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.