Leupeptin Hemisulfate

RT

426, 55

White powder

Leupeptin is a reversible competitive inhibitor of serine and thiol proteases that competes with substrate for the active site of the enzyme. It has been reported to inhibit calpain, cathepsin B and trypsin, for example. Leupeptin is often used for the inhibition of plasmin, trypsin, papain, kallikrein and cathepsin B. No inhibition found with pepsin, cathepsins A and D, elastase, renin or chymotrypsin. A typical working concentration is in the range of 10-100uM

Leupeptin has 3 tautomeric isomers which are separated by chromatography.

Synonyms:
Microbial Leupeptin; Acetyl-Leu-Leu-Arg-al; Leupeptin hemisulfate salt; N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt

CAS No:
103476-89-7

Molecular Formula:
C20H38N6O4•1/2H2SO4

Molecular Weight (Sulfate Form) (Ac-Leu-Leu-Argininal):
426.55

Appearance:
White powder

Optical Rotation:
-80.0° ± 2°

Water:
≤5%

Specific Activity (Trypsin/TAME):
~1.2ug/ml

Solubility:
Soluble in water, ethanol, acetic acid and DMF. Clear to v.slightly hazy solution at 50mg/mL in water.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Product is stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.