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Recombinant Human VEGFR2/KDR Protein (ECD, His Tag), HPLC-verified

Recombinant Human VEGFR2/KDR Protein (ECD, His Tag), HPLC-verified

Item no.	10012-H08H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Category	Oncology By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma By Organ Bladder Cancer
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
Conjugate/Tag	HIS
Purity	> 97 % as determined by SDS-PAGE
Sequence	Met1-Glu764
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD309,Flk-1,FLK1,VEGFR,VEGFR2
Similar products	KDR
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	KDR
Protein Construction	A DNA sequence encoding the extracellular domain of human VEGFR2 (NP_002244.1) (Met 1-Glu 764) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Using the Octet RED System, the affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-4 ug/mL in the presence of 10 ng/mL recombinant human VEGF165.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ala 20
Molecule Mass	The secreted recombinant human VEGFR2 consists of 756 amino acids and predicts a molecular mass of 84.6 kDa. The apparent molecular mass of rhVEGFR2 is approximatly 120-130 in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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