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Recombinant Human Lck Protein (GST Tag)

Recombinant Human Lck Protein (GST Tag)

Item no.	10043-H09B-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Oncology By Cell Type Hematology Lymphoma Myeloma Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
Conjugate/Tag	GST
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Pro509
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	IMD22,LSK,p56lck,pp58lck,YT16
Similar products	LCK
Available
Manufacturer - Conjugate / Tag	N-GST
Shipping Temperature	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	Lck
Protein Construction	A DNA sequence encoding the human LCK (NP_001036236.1) (Met 1-Pro 509) was fused with the GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. The specific activity was determined to be 70 nmol/min/mg using Poly(Glu, Tyr) 4:1 as substrate. 2. Measured by its ability to bind recombinant human CD3E in a functional ELISA.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human LCK/GST chimera consists of 734 amino acids and predicts a molecular mass of 84.4 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM GSH, 0.1mM EGTA, 0.1mM EDTA, 0.5mM PMSF, 10% glycerol

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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