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Recombinant Human GSK-3 beta/GSK3B Protein (His Tag)

Recombinant Human GSK-3 beta/GSK3B Protein (His Tag)

Item no.	10044-H07B-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Neuroscience Metabolism Cholesterol & Lipid Metabolism Aging & Neurological Disorders Neural Adhesion Molecules Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
Conjugate/Tag	HIS
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Thr433
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	10044-H07B
Similar products	GSK3B
Shipping Condition	Cool pack
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-His
Shipping Temperature	Blue ice shipping
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	GSK3B
Protein Construction	The amino acids corresponding to the full length of human GSK3B isoform 1 (NP_002084.2) (Met 1-Thr 433) was fused with a polyhistidine tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. The specific activity was determined to be 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate. 2. Immobilized His-GSK3B at 10 ug/ml (100 ul/well) can bind biotinylated human HG3C-CTNNB1 (cat:11279-H20B), EC50 of biotinylated human HG3C-CTNNB1 (cat:11279-H20B) is 0.15-0.35 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human GSK3B consists of 452 amino acids and predicts a molecular mass of 50.4 kDa. The apparent molecular mass of rhGSK3B is approximately 44-48 kDa in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10044-H07B-1-datasheet.pdf

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