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Recombinant Human IL-27 Protein (His Tag)

Recombinant Human IL-27 Protein (His Tag)

Item no.	10076-H08S-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 100 ug 2 mg 4 mg 5 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	CHO cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	IL-27, IL-27A, IL27A, IL27p28, IL30, p28
Similar products	IL27
Available
Manufacturer - Category	Protein
Molecule	IL27
Synonym	IL-27, IL-27A, IL27A, IL27p28, IL30, p28
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker was fused with a polyhistidine tag at the C-terminus.
Expressed Host	CHO Stable Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 ug/ml (100 ul/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.2-0.46 ug/ml. 2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 23-92 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Arg 21
Molecule Mass	The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, 10% glycerol
Accession	NP_005746.2 & NP_663634.2
Amino acid sequence	Met1-Lys229 & Phe29-Pro243
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 4 mg

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