Manufacturer |
Sino Biological
|
Category |
|
Type |
Proteins |
Specific against |
Human |
Amount |
4mg |
Item no. |
10082-HNAH-4 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Molecule |
MMP2 |
Synonym |
CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1 |
Storage |
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Protein Construction |
A DNA sequence encoding the native human MMP2 (NP_004521.1) (Met 1-Cys 660) was expressed and purified. |
Expressed Host |
Human Cells |
Purity |
> (74.7+17.3) % as determined by SDS-PAGE |
Bio-Activity |
1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 1, 000 pmoles/min/ug. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized human MMP2 at 10 ug/mL (100 ul/well) can bind human TIMP2/Fc (Cat: 10396-H01H). The EC50 of human TIMP2/Fc is 0.02 ug/mL. |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method |
Predicted N Terminal |
Ala 30 |
Molecule Mass |
The recombinant human MMP2 consists of 631 amino acids and migrates as an 72 kDa band as predicted in SDS-PAGE under reducing conditions. |
Formulation |
Lyophilized from sterile 0.05 % Brij-35, 150 mM NaCl, 5 mM CaCl2, 50 mM Tris, pH 7.5. |
Accession |
NP_004521.1 |
Amino acid sequence |
Met1-Cys660 |
TAG |
Native |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.