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Recombinant Human CD34 Protein (hFc Tag), HPLC-verified

Recombinant Human CD34 Protein (hFc Tag), HPLC-verified

Item no.	10103-H02H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug 500 ug
Category	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	Fc
Purity	> (67.1+28.7) % as determined by SDS-PAGE
Sequence	Met1-Thr290
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	10103-H02H
Similar products	CD34
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-human IgG1-Fc
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CD34
Protein Construction	A DNA sequence encoding the extracellular domain of human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) was fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to human CD34 coated plates (0.8 ug/ml, 100 ul/well), approximately >40 % will adhere after one hour at 37 C.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ser 32
Molecule Mass	The recombinant human CD34/Fc is a disulfide-linked homodimer generated after removal of the signal peptide. The reduced monomer consists of 497 amino acids and has a predicted molecular mass of 54 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is 116 and 96 kDa due to different glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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