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Recombinant Human CHL-1/L1CAM-2 Protein (His Tag), HPLC-verified

Recombinant Human CHL-1/L1CAM-2 Protein (His Tag), HPLC-verified

Item no.	10143-H08H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug 500 ug
Category	Oncology Neuroscience Neural Adhesion Molecules By Cell Type Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
Conjugate/Tag	HIS
Purity	> 95 % as determined by SDS-PAGE
Sequence	Met1-Gln1080
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CALL,L1CAM2
Similar products	CHL1
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CHL1
Protein Construction	A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CHL1 coated plates (0.8 ug/ml and 100 ul/well), approximately 40%-60% will adhere specifically after 60 minutes at 37C.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ile 25
Molecule Mass	The recombinant human CHL1 consists of 1067 amino acids after removal of the signal peptide and predicts a molecular mass of 120 kDa. As a result of glycosylation, the apparent molecular mass of rhCHL1 is approximately 160-180 kDa in SDS-PAGE under non-reduced conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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