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Recombinant Human HER3/ERBB3 Protein (aa 730-1065, His & GST Tag)

Recombinant Human HER3/ERBB3 Protein (aa 730-1065, His & GST Tag)

Item no.	10201-H20B1-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Astrocytoma By Organ Bladder Cancer Neuronal Cell Markers
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
Conjugate/Tag	GST, HIS
Purity	> 82 % as determined by SDS-PAGE
Sequence	Pro730-Ser1065
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	c-erbB-3,c-erbB3,EEBB3,ErbB-3,erbB3-S,HER3,LCCS2,MDA-BF-1,p180-ErbB3,p45-sErbB3,p85-sErbB3
Similar products	ERBB3
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-GST & His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	ERBB3
Protein Construction	A DNA sequence encoding the cytoplasmic domain (Pro 730-Ser 1065) of human ErbB3 (NP_001973.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	1. No Kinase Activity 2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST (Cat:10201-H20B1) bound to human NRG1 (aa 2-224) (Cat:11609-H04H) was 20 nM.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 1mM GSH

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10201-H20B1-1-datasheet.pdf

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