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Human CD4 / LEU3 Protein (His & Fc Tag)

Human CD4 / LEU3 Protein (His & Fc Tag)

Item no.	10400-H03H-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 2 mg 4 mg
Category	Neuroscience Aging & Neurological Disorders Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD4mut
Similar products	CD4
Available
Molecule	CD4
Synonym	CD4mut
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the human CD4 (NP_000607.1) extracellular domain (Met 1-Trp 390) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Purity	> 92 % as determined by SDS-PAGE
Bio-Activity	1. Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 x 10E4 cells/well areadded to CD4-Fch coated plates (1.25ug/mL and 100uL/well), approximately >50% will adhere specifically after 30 minutes at 37C. 2. Measured by its binding ability in a functional ELISA. Immobilized human CD4 at 10 ug/mL (100 uL/well) can bind biotinylated GP140-His (Tri) (Cat: 11677-V08H) with a linear range of 0.16-1.25ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Lys 26
Molecule Mass	The recombinant human CD4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 612 amino acids and has a predicted molecular mass of 68.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD4/Fc monomer is approximately 75 kDa due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_000607.1
Amino acid sequence	Met1-Trp390
TAG	C-human IgG1-Fc & His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 4 mg

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