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Human TRAIL R1 / CD261 / TNFRSF10A Protein (His & Fc Tag)

Human TRAIL R1 / CD261 / TNFRSF10A Protein (His & Fc Tag)

Item no.	10408-H03H-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 2 mg 4 mg
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Apoptosis
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	APO2, CD261, DR4, MGC9365, TNFRSF10A, TRAILR-1, TRAILR1
Similar products	TNFRSF10A
Available
Molecule	TNFRSF10A
Synonym	APO2, CD261, DR4, MGC9365, TNFRSF10A, TRAILR-1, TRAILR1
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the human TNFRSF10A (NP_003835.2) extracellular domain (Met 1-Asn 239) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Purity	> 98 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized human TNFSF10 at 10 ug/ml (100 ul/well) can bind human TNFRSF10A Fc Chimera with a linear range of 0.625-20 ng/ml. 2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L?929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 5-20 ng/ml in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ala 109
Molecule Mass	The recombinant human TNFRSF10A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 378 amino acids and has a predicted molecular mass of 42 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRSF10A/Fc monomer migrates with an apparent molecular mass of 47 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_003835.2
Amino acid sequence	Met1-Asn239
TAG	C-human IgG1-Fc & His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 4 mg

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