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Recombinant Human c-MET/HGFR Protein (ECD, His & hFc Tag), HPLC-verified

Recombinant Human c-MET/HGFR Protein (ECD, His & hFc Tag), HPLC-verified

Item no.	10692-H03H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 100 ug 2 mg 4 mg
Category	Oncology Neuroscience Targets for Rodent Neurosciences By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Mesothelioma
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	AUTS9, c-Met, DFNB97, HGFR, RCCP2
Similar products	MET
Available
Manufacturer - Category	Protein
Molecule	MET
Synonym	AUTS9, c-Met, DFNB97, HGFR, RCCP2
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human c-Met (NP_000236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 ug/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM .
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 25
Molecule Mass	The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved alpha and beta subunits. Each alpha and beta together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (alpha =32.5 + Fc tagged beta=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_000236
Amino acid sequence	Met1-Thr932
TAG	C-human IgG1-Fc & His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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