Recombinant Human FGFR2 Protein (aa 400-821, His & GST Tag)

N-GST & His

Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the human FGFR2 (NP_000132.3) cytoplasmic domain (Met 400-Thr 821) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

1. The specific activity was determined to be 28 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR2 (aa 400-821) (Cat:10824-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF acidic (Cat:10013-HNAE) with a linear range of 15.6-250 ng/ml.
3. Measured by its binding ability in a functional ELISA. Immobilized recombinant recombinant human FGFR2 (aa 400-821) (Cat:10824-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF basic (Cat:10014-HNAE) with a linear range of 0.16-1.25 ug/ml.

Gln 26

Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly