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Recombinant Human PLA2G7/PAF-AH Protein (His Tag)

Recombinant Human PLA2G7/PAF-AH Protein (His Tag)

Item no.	10848-H08H-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 10 ug 2 mg 20 ug 4 mg 50 ug
Category	Neuroscience Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Peptides & Proteins Recombinant Proteins Enzymes Fatty Acid Catabolism
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Purity	> 88 % as determined by SDS-PAGE
Sequence	Met1-Asn441
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	LDL-PLA2,LP-PLA2,PAFAD,PAFAH
Similar products	PLA2G7
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	PLA2G7
Protein Construction	A DNA sequence encoding the human PLA2G7 (Q13093-1) precursor (Met 1-Asn 441) was expressed, with a C-terminal polyhistidine tag.
Expressed Host	Human Cells
Bio-Activity	Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF), in the presence of 5, 5’Dithiobis(2-nitrobenzoic acid) (DTNB). The specific activity is >5000 pmoles/min/ug.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Phe 22
Molecule Mass	The secreted recombinant human PLA2G7 comprises 431 amino acids with a predicted molecular mass of 49.2 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM NaAc, 150mM NaCl, 10% glycerol, pH 5.0

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10848-H08H-1-datasheet.pdf

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