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Recombinant Human SIRP alpha/SHPS-1 Protein (G75A, ECD, His Tag), HPLC-verified

Recombinant Human SIRP alpha/SHPS-1 Protein (G75A, ECD, His Tag), HPLC-verified

Item no.	11612-H08H-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 100 ug 2 mg 4 mg 50 ug
Category	Neuroscience Targets for Rodent Neurosciences Neural Adhesion Molecules Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human (Homo sapiens)
Conjugate/Tag	HIS
Purity	> 98 % as determined by SDS-PAGE
Sequence	Met1-Arg370
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	BIT,CD172A,MFR,MYD-1,P84,PTPNS1,SHPS1,SIRP,SIRP alpha
Similar products	SIRPA
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	SIRPA
Protein Construction	A DNA sequence encoding the human SIRPA (NP_542970.1) extracellular domain (Met 1-Arg 370) was expressed, fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 ug/ml (100 ul/well) can bind human CD47-Fc (Cat:12283-H02H), The EC50 of human CD47-Fc (Cat:12283-H02H) is 10.1-23.5 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 31
Molecule Mass	The recombinant human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSIRPA is approximately 45-50 kDa due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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