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Human A2aR -Nanodisc Protein(His & Flag Tag)

Human A2aR -Nanodisc Protein(His & Flag Tag)

Item no.	12307-H18H-NA-20
Manufacturer	Sino Biological
Amount	20 ug
Quantity options	100 ug 20 ug 500 ug
Category	Oncology By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Conjugate/Tag	FLAG, HIS
Purity	≥ 95 % as determined by SDS-PAGE.
Dry ice	Yes
Sequence	Pro2-Leu208 & Glu219-Ala316
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Dry ice
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-Flag, C-His
Shipping Temperature	Dry ice
Storage Conditions	-20C
Molecular Weight	The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions. FP6 predicts a molecular mass of 11.83 kDa
Antigen molecule name	AA2AR
Buffer	Supplied as sterile 10mM Hepes, 50mM NaCl, pH 7.5.
Protein Construction	A DNA sequence encoding the human AA2AR (P29274) (Pro2-Leu208 & Glu219-Ala316) (TM domains 1-7) was expressed and linked by a Fusion Protein, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Bio-Activity	1.Immobilized Recombinant Human ADORA2A-Nanodisc Protein (Cat: 12307-H18H-NA) at 5 μg/mL (100 μL/well) can bind AT004-Derived Anti-Human ADORA2A Monoclonal Antibody, Mouse IgG2a, the EC50 is 2-6 ng/mL. 2.Loaded Human A2aR -Nanodisc Protein, His & Flag Tag (Cat. No. 12307-H18H-NA) on AR2G Biosensor via Anti-Fusion Protein Monoclonal antibody, Mouse IgG2a, can bind AT004-Derived Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a with an affinity constant of 0.847nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Loaded AT004-Derived Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a on ProA Biosensor, can bind Human A2aR -Nanodisc Protein, His & Flag Tag (Cat. No. 12307-H18H-NA) with an affinity constant of 2.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
N-terminus	Asp

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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