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Recombinant Porcine (pig) FABP3 Protein, HEK293

Recombinant Porcine (pig) FABP3 Protein, HEK293

Item no.	230-30146-100
Manufacturer	Raybiotech
Amount	100 ug
Quantity options	10 ug 100 ug 200 ug 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized
Applications	WB, IP, ELISA, Dot
Specific against	other
Host	Human, HEK293
Conjugate/Tag	HIS
Purity	>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Animal Proteins
Manufacturer - Conjugate / Tag	N-terminal his-tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of 15 kDa. Due to the abundant glycosylation, it migrates as approximately 18 kDa extra protein band (beside 15 kDa band) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one single 15 kDa band. See deglycosylation analysis in SDS-PAGE image.
Gene Symbols	FABP3
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region	Val2-Ala133
Protein Name & Synonyms	Fatty acid-binding protein, heart, Fatty Acid Binding Protein 3, Mammary-Derived Growth Inhibitor, Fatty Acid Binding Protein 3, Muscle And Heart, Heart-Type Fatty Acid-Binding Protein, Muscle Fatty Acid-Binding Protein, Fatty Acid Binding Protein 11, FABP11, H-FABP, M-FABP, MDGI, Fatty Acid Binding Protein 3, Muscle And Heart (Mammary-Derived Growth Inhibitor), Fatty Acid-Binding Protein 3, O-FABP.
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by BCA protein assay (lot specific)
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein under reducing conditions.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.Lane 5: deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.