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Recombinant SARS-CoV-2, S1 Subunit Protein (RBD), Biotinylated

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD), Biotinylated

Item no.	230-30162B-100
Manufacturer	Raybiotech
Amount	100 ug
Quantity options	100 ug 1000 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins Western Blot ELISA Immunostaining Lateral Flow
Type	Proteins Recombinant
Format	Liquid
Applications	WB, ELISA, LF
Specific against	SARS-CoV-2
Host	HEK293 cells
Conjugate/Tag	Biotin, HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, binding assay, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Viral Proteins\|COVID-19\|Proteins\|Spike Protein
Manufacturer - Conjugate / Tag	C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Description	Biotin-conjugated recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
UNSPSC Code	12352202
Formulation	Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	Arg319-Phe541
Protein Name & Synonyms	Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Lot specific (see the label on the vial), determined by BCA protein assay

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.