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Recombinant SARS-CoV-2 S2 Subunit Protein (full length)

Recombinant SARS-CoV-2 S2 Subunit Protein (full length)

Item no.	230-30163-10
Manufacturer	Raybiotech
Amount	10 ug
Quantity options	10 ug 50 ug
Category	Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Liquid
Specific against	SARS-CoV-2
Host	HEK293 cells
Conjugate/Tag	HIS
Purity	>90 %
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Cool pack
Available
Manufacturer - Category	Proteins\|Recombinant Proteins\|Viral Proteins\|COVID-19\|Proteins\|Spike Protein
Manufacturer - Conjugate / Tag	C-terminal his-tag
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of ∼60 kDa. Due to the abundant glycosylation, it migrates as approximately 80 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Description	Recombinant SARS-CoV-2 S2 Subunit Protein with C-terminal tag, derived from the transfected human HEK293 cells. Datasheet
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
UNSPSC Code	12352202
Formulation	Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	Met697-Pro1213
Protein Name & Synonyms	Spike protein, S Protein, S2 Subunit
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Lot specific (see the label on the vial), determined by BCA protein assay
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (ˆ¼60 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼80 kDa) was glycosylated.Lane 1: Protein standard ladder (kDa)Lane 2: Untreated protein (ˆ¼80 kDa, red arrow) under reducing conditionsLane 3: Partially cleaved protein (ˆ¼60 kDa, green arrow) with deglycosylation enzymes under native conditionsLane 4: Fully cleaved protein (ˆ¼60 kDa, green arrow) with deglycosylation enzymes under reducing conditions
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.