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Recombinant SARS-CoV-2 S1 Subunit Protein (RBD), mutant N501Y, Biotinylated

Recombinant SARS-CoV-2 S1 Subunit Protein (RBD), mutant N501Y, Biotinylated

Item no.	230-30184B-500
Manufacturer	Raybiotech
Amount	500 ug
Quantity options	100 ug 50 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Type	Proteins Recombinant
Format	Liquid
Applications	WB, IP, ELISA, Dot
Specific against	SARS-CoV-2
Host	HEK293 cells
Conjugate/Tag	Biotin, HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Viral Proteins\|COVID-19\|Proteins\|Spike Protein\|Spike Protein Variants
Manufacturer - Conjugate / Tag	C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of ∼25 kDa. Due to the abundant glycosylation, it migrates as approximately ∼30 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one major ∼25 kDa band. See deglycosylation analysis image below.
Description	Biotin-conjugated recombinant SARS-CoV-2 S1 Subunit Protein (RBD) mutant N501Y with C-terminal His-tag, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	S
UNSPSC Code	12352202
Formulation	Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	Arg319-Phe541. Amino acid Asn (N501) was mutated to Tyr (Y501).
Protein Name & Synonyms	Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Lot specific (see the label on the vial), determined by BCA protein assay
Endotoxin Level	< 0.5 EU per µg of the protein as determined by the LAL method

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.