Trypsin, 0.22µ Filtered

Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglu, Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglutination procedures Sample preparation for flow cytometric DNA analysis Tryptic mapping Fingerprinting and sequencing work Environmental monitoring Reduction of cell density in tissue culture Subculturing cells Cleavage fusion proteins Generating glycopeptides from purified glycoproteins (White and White 1997)

Bovine Pancreas

2-8°C

Trypsin chromatographically purified, diafiltered filtered thorugh a 0.22 micron pore size membrane and lyophilized in sterile vials. This product is not tested for pyrogenicity.

≥180 units per mg protein (at least 10, 350 BAEE/3, 450 USP/NF u/mg P)

Trypsinogen: pH 9.3 (Walsh and Neurath 1964) Trypsin: pH 10.5 (Cunningham 1954).