Trypsin, Purified, Sequencing Grade II

Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglu, Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglutination procedures Sample preparation for flow cytometric DNA analysis Tryptic mapping Fingerprinting and sequencing work Environmental monitoring Reduction of cell density in tissue culture Subculturing cells Cleavage fusion proteins Generating glycopeptides from purified glycoproteins (White and White 1997)

Bovine Pancreas

-20°C

Bovine trypsin that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity and extensively purified to remove autolysis products, resulting in a highly stable trypsin product resistant to autolysis while retaining specificity. Supplied as a lyophilized powder.

≥150 units per mg protein (at least 8, 625 BAEE/2875 USP/NF units per mg protein)

Trypsinogen: pH 9.3 (Walsh and Neurath 1964) Trypsin: pH 10.5 (Cunningham 1954).

Yes