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Mouse CD86 / B7-2 Protein (His & Fc Tag)

Mouse CD86 / B7-2 Protein (His & Fc Tag)

Item no.	50068-M03H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 2 mg 4 mg
Category	Neuroscience Neural Lineage Markers Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	B7, B7-2, B7.2, B70, Cd28l2, CLS1, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2
Similar products	CD86
Available
Molecule	CD86
Synonym	B7, B7-2, B7.2, B70, Cd28l2, CLS1, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Glu 245) of mouse CD86 (NP_062261.3) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Expressed Host	Human Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA . Immobilized human CD28 at 20 ug/ml (100 ul/well) can bind mouse CD86 Fc Chimera with a linear ranger of 6.4-160 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Val 24
Molecule Mass	The recombinant mouse CD86/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 470 amino acids and has a predicted molecular mass of 53.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86/Fc monomer is approximately 70-80 kDa due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_062261.3
Amino acid sequence	Met1-Glu245
TAG	C-human IgG1-Fc & His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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