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Recombinant Mouse IFN gamma Protein (His Tag)

Recombinant Mouse IFN gamma Protein (His Tag)

Item no.	50709-M08H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 100 ug 2 mg 4 mg
Category	Neuroscience Neuroinflammation Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Ifg, IFN-g
Similar products	IFNG
Available
Manufacturer - Category	Protein
Molecule	IFNG
Synonym	Ifg, IFN-g
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the mouse IFNG (P01580) (Met 1-Cys 155) was expressed, fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Purity	> 75 % as determined by SDS-PAGE
Bio-Activity	1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His (cat:50709-M08H)at 10 ug/ml (100 ul/well) can bind mouse IFNGR1-Fc (Cat:50705-M02H). The EC50 of mouse IFNGR1-Fc (Cat:50705-M02H) is 58.2-135.9 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	His 23
Molecule Mass	The recombinant mouse IFNG comprises 144 amino acids and has a calculated molecular mass of 17 kDa. As a result of glycosylation, the apparent molecular mass of rn IFNG is approximately 21 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	P01580
Amino acid sequence	Met1-Cys155
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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