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Recombinant Mouse Cyclin E Protein (His & GST Tag)

Recombinant Mouse Cyclin E Protein (His & GST Tag)

Item no.	50896-M20B-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg
Category	Oncology Neuroscience Neural Lineage Markers By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Proliferation By Organ Bladder Cancer Colorectal Cancer Testicular & Prostate Cancer
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Baculovirus-Infected Insect Cells
Conjugate/Tag	GST, HIS
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Glu408
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	AW538188,CycE1
Similar products	CCNE1
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-GST & His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CCNE1
Protein Construction	A DNA sequence encoding the mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized mouse CCNE1 (Cat:50896-M20B) at 10 ug/ml (100 ul/well) can bind biotinylated human CDK4 (Cat:10732-H09B), The EC50 of biotinylated human CDK4 (Cat:10732-H09B) is 48.0-114.0 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant mouse CCNE1/GST chimera consists of 645 amino acids and has a calculated molecular mass of 74.8 kDa. The recombinant protein migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM Nacl, 3mM DTT, 0.5mM GSH, 10% gly, pH 8.0.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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50896-M20B-1-datasheet.pdf

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