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Recombinant Mouse IL-5 Protein (His Tag)

Recombinant Mouse IL-5 Protein (His Tag)

Item no.	51068-M08B-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Il-5
Similar products	IL5
Available
Manufacturer - Category	Protein
Molecule	IL5
Synonym	Il-5
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the mouse IL5 (P04401) (Met 1-Gly 133) was expressed, with a C-terminal polyhistidine tag.
Expressed Host	Baculovirus-Insect Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse IL5-his at 5 ug/ml (100 ul/well) can bind biotinylated human IL5Ra-his with a linear range of 32-4000 ng/mL. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met 21
Molecule Mass	The recombinant mouse IL5 consists of 123 amino acids and has a calculated molecular mass of 14.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions, and the homodimer form could also be seen.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% gly
Accession	P04401
Amino acid sequence	Met1-Gly133
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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