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Recombinant Rat E-Selectin/CD62E Protein (His Tag), HPLC-verified

Recombinant Rat E-Selectin/CD62E Protein (His Tag), HPLC-verified

Item no.	80033-R08H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 100 ug 2 mg 200 ug 4 mg
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Signaling Neural Adhesion Molecules By Cell Type Hematology Leukemia Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion
Type	Proteins
Specific against	Rat (Rattus norvegicus)
Conjugate/Tag	HIS
Purity	> 97 % as determined by SDS-PAGE
Sequence	Met1-Pro494
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	80033-R08H
Similar products	SELE
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	SELE
Protein Construction	A DNA sequence encoding the rat SELE (P98105-1) extracellular domain (Met 1-Pro 494) was expressed, fused with a polyhistidine tag at the C-terminus.
Expressed Host	Human Cells
Bio-Activity	Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to rat E Selectin/Fc Chimera coated plates (2 ug/mL, 100 uL/well), approximately 30%-60% will adhere after 1 hour at 37C. Optimal dilutions should be determined by each laboratory for each application.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Trp 22
Molecule Mass	The recombinant rat SELE comprises 484 amino acids and predicts a molecular mass of 53 kDa. The apparent molecular mass of the rat SELE is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 2 mg

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