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Recombinant Cynomolgus RANKL/TNFSF11 Protein (hFc Tag)

Recombinant Cynomolgus RANKL/TNFSF11 Protein (hFc Tag)

Item no.	90301-C01H-1
Manufacturer	Sino Biological
Amount	1 mg
Quantity options	1 mg 100 ug 2 mg 20 ug 4 mg 5 ug
Category	Oncology Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines By Organ Lymphatic System Cancer
Type	Proteins
Specific against	Monkey (Cynomolgus, Simian)
Host	Human
Conjugate/Tag	Fc
Purity	> 85 % as determined by SDS-PAGE
Sequence	Gly136-Asp317
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	90301-C01H
Similar products	TNFSF11
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	N-human IgG1-Fc
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	TNFSF11
Protein Construction	A DNA sequence encoding the cynomolgus TNFSF11 (G7PW41) (Gly136-Asp317) was expressed with the Fc region of human IgG1 at the N-terminus.
Expressed Host	Human Cells
Bio-Activity	1. Immobilized Cynomolgus S4-Fc3L3-TNFSF11 at 10 ug/ml (100 ul/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 7.94-18.52 ng/ml. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 0.1-0.4 ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu
Molecule Mass	The recombinant cynomolgus TNFSF11 comprises 442 amino acids and has a calculated molecular mass of 48.9 KDa. The apparent molecular mass of the protein is approximately 55 KDa in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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