Human GFRalpha1-Fc Chimera

Proteins

Israel

Storage as Solution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Lyophilized

An Inhibitor of GDNF Activity

An Inhibitor of GDNF Activity

12352202

4 - 10 µg/ml in the presence of 10 ng/ml of human GDNF.

Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.

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GDNF (Glial cell line neurotrophic factor) is a neurotrophic factor that increases the survival of midbrain dopaminergic neurons and is essential for the development of the kidneys and the enteric nervous systeM This factor is a glycosylated disulfide-bonded homodimer and is distantly related to the growth factor-β super family1.GDNF binds with high affinity in GDNF responsive cells to the Glycosyl-phosphatidylinositol (GPI) linked receptor known as GDNFR or GFRα12 and also requires the presence of the Ret protein tyrosine kinase for the continuation of signal transduction3.GFRα1 is an anchored cell surface molecule and was mapped to the human chromosome 10. It was found to have an integral role in RET proto oncogene-mediated signaling thus being linked to disorders such as Hirschsprung's disease in case of a loss of function mutation and various cancers in gain of function mutations4.Human GFRα1-Fc Chimera inhibits GDNF-induced phosphorylation of ERK1/2 MAPK and Akt in SH-5Y5Y cells and can prove as an important ligand in the research of GDNF associated diseases.