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Cynomolgus CD73/NT5E Protein (CD7-CM173)

Cynomolgus CD73/NT5E Protein (CD7-CM173)

Item no.	CD7-CM173-500UG
Manufacturer	KactusBio
Amount	500 ug
Quantity options	100 ug 500 ug
Category	Oncology Neuroscience Molecular Targets for Neuroscience By Cell Type Peptides & Proteins Recombinant Proteins Carcinom
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Monkey (Cynomolgus, Simian)
Host	HEK293
Conjugate/Tag	HIS
Purity	> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Formula	Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Sequence	Trp27-Cys554
NCBI	EHH53214.1
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD73,NT5E,NTE,5' nucleotidase (CD73),5'-NT,5-NT,E5NT,EC 3.1.3.5,eN,eNT,NT,NT55'-nucleotidase,NT5,NTE,CALJA
Shipping Condition	Room temperature
Available
Specificity	Cynomolgus
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	CD73/NT5E
Manufacturer - Conjugate / Tag	C-His
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Description	Recombinant Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Cys554.
Background	CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CD7-CM173-500UG-datasheet.pdf

CD7-CM173-500UG-safety-datasheet.pdf