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Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein (His & GST Tag)

Recombinant Human AMPK (G1/B2/A2) Heterotrimer Protein (His & GST Tag)

Item no.	CT006-H0907B-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Oncology Metabolism By Cell Type Hematology Lymphoma Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Status Autophagy
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	5' adenosine monophosphate-activated protein kinas, 5' AMP-activated protein kinase, AMPK, beta 1 non-catalytic subunit and gamma 1 non-catal
Similar products	AMPK
Available
Manufacturer - Category	Protein
Shipping method	Blue ice shipping
Molecule	AMPK
Synonym	5' adenosine monophosphate-activated protein kinas, 5' AMP-activated protein kinase, AMPK, beta 1 non-catalytic subunit and gamma 1 non-catal
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Expressed Host	Baculovirus-Insect Cells
Purity	> 90 % as determined by SDS-PAGE
Bio-Activity	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met & Met & His
Molecule Mass	The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 200mM NaCl, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 10% gly, 1mM GSH, pH 7.4
Accession	P54619 & Q9Y478 & P54646
Amino acid sequence	Met1-Pro331 & Met1-Ile270 & Met1-Arg552
TAG	N-GST & N-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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