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Recombinant Human IL-12 Protein (His Tag), HPLC-verified

Recombinant Human IL-12 Protein (His Tag), HPLC-verified

Item no.	CT011-H08H-2
Manufacturer	Sino Biological
Amount	2 mg
Quantity options	1 mg 2 mg 4 mg 50 ug 500 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CLMF, CLMF2, IL-12A, IL-12B, IL12, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
Similar products	IL12A & IL12B
Available
Manufacturer - Category	Protein
Molecule	IL12A & IL12B
Synonym	CLMF, CLMF2, IL-12A, IL-12B, IL12, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Expressed Host	Human Cells
Purity	> 95 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Arg 23 & Ile 23
Molecule Mass	The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_000873.2 & NP_002178.2
Amino acid sequence	Met1-Ser219 & Met1-Ser328
TAG	C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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