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Recombinant Human CDK7 & CCNH & MNAT1 Protein (His Tag)

Recombinant Human CDK7 & CCNH & MNAT1 Protein (His Tag)

Item no.	CT020-H07B-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling Peptides & Proteins Recombinant Proteins By Organ Pancreatic Cancer Nervous System Cancer Astrocyte Markers
Type	Proteins
Specific against	Human (Homo sapiens)
Host	Baculovirus-Infected Insect Cells
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CAK1, CDKN7, HCAK, MO15, p39MO15, STK1
Similar products	CDK7 & CCNH & MNAT1
Available
Manufacturer - Category	Protein
Shipping method	Blue ice shipping
Molecule	CDK7 & CCNH & MNAT1
Synonym	CAK1, CDKN7, HCAK, MO15, p39MO15, STK1
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human CDK7 (P50613) (Ala 2-Phe 346) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNH (P51946) (Tyr 2-Leu 323) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. A DNA sequence encoding the human MNAT1 (P51948) (Asp 2-Ser 309) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Expressed Host	Baculovirus-Insect Cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	The specific activity was determined to be 18 nmol/min/mg using MBP as substrate.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	His & His & His
Molecule Mass	The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25, 38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile PBS, pH 7.4
Accession	P50613 & P51946 & P51948
Amino acid sequence	Ala2-Phe346 & Tyr2-Leu323 & Asp2-Ser309
TAG	N-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CT020-H07B-4-datasheet.pdf

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Amount: 4 mg

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