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Recombinant Mouse IL-12 Protein (His & hFc Tag)

Recombinant Mouse IL-12 Protein (His & hFc Tag)

Item no.	CT024-M0208H-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Category	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Il-12a, Il-12b, IL-12p35, Il-12p40, Il12p40, Ll12a, p35, p40
Similar products	IL12A & IL12B
Available
Manufacturer - Category	Protein
Molecule	IL12A & IL12B
Synonym	Il-12a, Il-12b, IL-12p35, Il-12p40, Il12p40, Ll12a, p35, p40
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Expressed Host	Human Cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12RB2-His at 10 ug/ml (100 ul/well) can bind Mouse IL12A & IL12B Heterodimer Protein (Cat:CT024-M0208H). The EC50 of Mouse IL12A & IL12B Heterodimer Protein (Cat:CT024-M0208H) is 50.2-117.2 ng/ml. 2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-1 ng/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Arg 23 & Met 23
Molecule Mass	The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	P43431 & P43432
Amino acid sequence	Met1-Ala215 & Met1-Ser335
TAG	C-human IgG1-Fc & C-His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CT024-M0208H-4-datasheet.pdf

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Amount: 4 mg

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