« Back
Home /
Recombinant Mouse CD3D & CD3E Heterodimer Protein (Fc-Flag & Fc-His Tag)

Recombinant Mouse CD3D & CD3E Heterodimer Protein (Fc-Flag & Fc-His Tag)

Item no.	CT025-M2303H-4
Manufacturer	Sino Biological
Amount	4 mg
Quantity options	1 mg 2 mg 20 ug 4 mg 50 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Host	Human
Conjugate/Tag	FLAG, HIS, Fc
Purity	> 90 % as determined by SDS-PAGE
Sequence	Met1-Ala105 & Met1-Asp108
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	T3d
Similar products	CD3D & CD3E
Available
Manufacturer - Type	Protein
Manufacturer - Conjugate / Tag	C-human IgG1-Fc & Flag & C-human IgG1-Fc & His
Shipping Temperature	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Storage Conditions	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Antigen molecule name	CD3D & CD3E
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
Expressed Host	Human Cells
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Phe 22 & Asp 22
Molecule Mass	The recombinant heterodimer of mouse CD3D/CD3E comprises 670 (333+337) amino acids and has a calculated molecular mass of 76 (38+38? kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

CT025-M2303H-4-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 4 mg

available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question