Recombinant human CDC42 protein

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA

Signal Transduction

< 1 EU per 1ug of protein (determined by LAL method)

CDC42, ATGP0395-10ug, ATGP0395-20ug, ATGP0395-50ug, ATGP0395-100ug, ATGP0395-250ug, ATGP0395-500ug, ATGP0395-1mg, ATGP0395-10, ATGP0395-20, ATGP0395-50, ATGP0395-100, ATGP0395-250, ATGP0395-500, ATGP0395-1