Manufacturer |
ProSci
|
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Format |
Liquid |
Applications |
WB, ELISA, MS |
Amount |
0.05 mg |
Item no. |
PRS-XW-RP3020-0.05mg |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Applications |
This recombinant protein can be used for WB, ELISA, MS. |
Applications |
This recombinant protein can be used for WB, ELISA, MS. |
Buffer |
10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3 |
Storage Conditions |
Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Disclaimer |
For research use only. |
Predicted Molecular Weight |
26 kDa (Calculated) |
Purity |
95% |
Background |
This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of a hexamer of three A and three B subunits plus the C, D, and E subunits. It contains the ATP catalytic site. The protein is known as the E subunit and is found ubiquitously. |
1St Image Caption3 |
SDS PAGE: Analysis of ATP6V1E1 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining. |
Background References 1 |
Baud, V.; Mears, A. J.; Lamour, V.; Scamps, C.; Duncan, A. M. V.; McDermid, H. E.; Lipinski, M. : The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22. Hum. Molec. Genet. 3: 335-339, 1994. |
Accession # |
NP_001687 |
Ncbi Gene Id # |
529 |
Ncbi Official Symbol |
ATP6V1E1 |
Ncbi Official Full Name |
ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P36543 |
Sequence |
aa. 1-226 |
Biological Activity |
N/A |
Protein Gi# |
4502317 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.