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Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein

Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein

Item no.	RP00166-20ug
Manufacturer	Abclonal
Amount	20 ug
Quantity options	100 ug 200 ug 20 ug 500 ug 50 ug
Category	Oncology Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Apoptosis
Type	Proteins Recombinant
Specific against	Human (Homo sapiens)
Host	Human
Purity	> 90% by SDS-PAGE.
NCBI	TNFRSF12A/TWEAKR/CD266
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD266,FN14,TWEAKR,TNFRSF12A,FN14,TWEAKR
Similar products	CD266, FN14, TWEAKR
Shipping Condition	Cool pack
Available
Manufacturer - Applications	< 0.1 EU/μg of the protein by LAL method.
Manufacturer - Category	Proteins
Shipping Temperature	ice pack
Storage Conditions	Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt. \|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Protein Weight	32.30 kDa
Description	Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu28-Trp79) of human TNFRSF12A/FN14/TWEAKR (Accession #NP_057723.1) fused with an Fc, 6×His tag at the C-terminus.
Background	Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Human and mouse TNFRSF12A share 82% aa sequence identity. TNFRSF12A transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated TNFRSF12A expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.
Manufacturer - Cross Reactivity	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Immunogen	Glu28-Trp79
Route	C-hFc&His
Manufacturer - Research Area	Bio-Markers & CD Antigens, TNF family, Biosimilar Drug Targets
Revised name	CD266, FN14, TWEAKR
Antigen Seq	EQAPGTAPCSRGSSWSADLDKCMDCASCRARPHSDFCLGCAAAPPAPFRLLW
Bioactivity	1. Measured by its ability to inhibit TWEAK-induced apoptosis in HT-29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 2-12 μg/mL in the presence of 1 μg/mL recombinant human TWEAK.\|2. Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0. 1-2. 3 ng/mL.\|3. Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.
Protein Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Expected Protein Size	32.30 kDa
Gene Symbol	TNFRSF12A/TWEAKR/CD266

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20 ug

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Delivery expected until 10/2/2025

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