Comparison

Human Cytokine Array C1

Manufacturer Raybiotech
Category
Type Array
Specific against Human
Amount 2 Sample Kit
Item no. AAH-CYT-1-2
Targets IL2 ;; IL5 ;; IL6 ;;CCL2;;CCL5;;CCL8;;CXCL8;;IL10;;IL13;;IL15;;IL1A;;TGFB1
eClass 6.1 32161000
eClass 9.0 32161000
Available
Short description
RayBio C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
23
Target Names
GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MIG (CXCL9), RANTES (CCL5), TGF beta 1, TNF alpha, TNF beta (TNFSF1B)
Targets Detected
CCL2, CCL5, CCL7, CCL8, CXCL1, CXCL8, CXCL9, G-CSF, GM-CSF, GRO, GRO alpha, IFN-gamma, IL-1 alpha, IL-1 F1, IL-10, IL-13, IL-15, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, MARC, MCP-1, MCP-2, MCP-3, MIG, RANTES, TGF beta 1, TNF alpha, TNF beta, TNFSF1B
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
  • 1. Fisher, Bridget S., Carlos E. Estraño, and Judith A. Cole. "Modeling Long-Term Host Cell-Giardia Lamblia Interactions in an In Vitro Co-Culture System." PLoS One. NCBI, 03 Dec. 2013. Web.
  • 2. Caballero-Campo, Pedro, George L. Gerton, and Mariano G. Buffone. "A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis." Journal of Cellular Physiology. NCBI, 01 Jan. 2014. Web.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 2 Sample Kit
Available: In stock
available

Delivery expected until 4/25/2024 

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