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Human HSP Array C1

Human HSP Array C1

Manufacturer	Raybiotech
Category	Oncology Neuroscience Neuroinflammation Neural Mitochondrial Function Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding By Cell Type Carcinoma Sarcoma Cell Biology Cell Status Autophagy Arrays Antibody Arrays
Type	Assay
Specific against	Human
Amount	8 Sample Kit
Item no.	AAH-HSP-1-8
Targets	HSP90AA1;;HSPA4;;HSPA9;;HSPD1
eClass 6.1	32161000
eClass 9.0	32161000
Available
Short description	RayBio C-Series Human Heat Shock Protein Antibody Array Kit. Detects 9 Human heat shock proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected	9
Target Names	HSP10, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, GRP75, Ubiquitin+1
Targets Detected	GRP75, HSP10, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, Ubiquitin+1
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components	Human HSP Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer Protease Inhibitor Cocktail 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage	For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes a -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Membrane
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Chemiluminescence
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.