Human Cytokine Array Q640

Multiplexed Protein Detection
Biomarker Screening
Identifying Key Factors
Confirming Biological Process
Biomarker Validation
Validation of Antibody Array Results
Quantitative Protein Detection
Establishing Normal Range

IFN-gamma, TNF alpha, Angiopoietin-1, bFGF, Decorin, IGF-1, IL-10, IL-13, IL-15, IL-17A, IL-18, IL-2, IL-21, IL-4, LIF, VEGF-A, Cystatin C, EGFR, ErbB3, Galectin-3, GM-CSF, HGF, IGFBP-2, IL-12 p40, IL-6, MMP-8, PDGF-BB, RAGE, Resistin, TGF alpha, Trappin-2, IL-5, IL-16, ACE-2, AgRP, Angiopoietin-2, FGF-6, Growth Hormone, IGF-1 R, IGFBP-1, IGFBP-3, IL-11, IL-12 p70, Insulin, Leptin, Leptin R, M-CSF, MIF, MSP alpha/beta, Oncostatin M, PAI-1, PDGF-AA, PDGF-AB, Somatotropin, TGF beta 1, TIMP-1, TIMP-2, TNFRSF27, XEDAR, Albumin, Beta-2 Microglobulin, Clusterin, CXCL16, FABP1, L-FAP1, OPN, p53, Angiogenin, Angiostatin, EGF, GCSF, GRO alpha/beta/gamma, MMP-1, MMP-9, PLGF, uPAR, VEGF-D, VEGFR2, VEGFR3, Bcl-w, BIRC4, cIAP-2, HTRA2, IAP-3, IGF-2, IGFBP-4, IGFBP-5, IGFBP-6, Omi, XIAP, GDNF, TGF beta 2, TGF beta 3, Activin A, ADAM-9, ADAMTS-13, ALK-1, Alpha-fetoprotein, AMICA, Amphiregulin, Angiopoietin-4, APRIL, Artemin, AXL, BCAM, BDCA-3, BDNF, beta IG-H3, beta-NGF, BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-II, C5a, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calcitonin, Cathepsin L, Cathepsin S, CD10, CD138, CD14, CD163, CD200, CD23, CD97, CEA, CEACAM-1, CEACAM-5, Chemerin, CHI3L1, CNTF, CNTF R alpha, Contactin-1, CRIM1, Cripto-1, CRTAM, CSFR1, Cystatin A, Cystatin B, DAN, DKK-1, Dkk-3, Dkk-4, DLL1, Dtk, E-Cadherin, E-Selectin, EDA-A2, EDAR, Elafin, Endocan, Enolase 2, EphA1, EphA2, EphB4, EphB6, Epiregulin, Epo R, ErbB1, ErbB2, Erythropoietin R, ESAM, FABP2, FAP, Fc epsilon RII, Ferritin, Fetuin A, FGF-12, FGF-17, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-9, FIL1 delta, FIL1 epsilon, FIL1 eta, FIL1 zeta, FLRG, Flt-3 Ligand, Follistatin, FOLR1, FSH, Furin, Galectin-1, Galectin-7, Gas 1, GDF-15, GLP-1, Glucagon-like peptide 1, Glypican 5, GM-CSF R alpha, Growth Hormone R, HAI-1, HAI-2, HB-EGF, hCG beta, IFN-alpha/beta R2, IFN-gamma R1, IGF-2 R, IL-17B, IL-17C, IL-17F, IL-20, IL-22BP, IL-23, IL-24, IL-27, IL-3, IL-31, IL-34, IL-7, IL-9, Integrin alpha-5, Kallikrein 14, Kallikrein 5, Kallikrein 7, Kallistatin, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIF R alpha, LIMPII, Lipocalin-1, LOX-1, LRP-6, Lumican, Luteinizing hormone, LYVE-1, M-CSF R, Marapsin, MBL, Mer, Mesothelin, MFRP, MICA, MICB, Midkine, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, Neprilysin, Neuropilin-2, Neurturin, Nidogen-1, Notch-1, Nr-CAM, NSE, NT-3, NT-4, OLR1, P-Cadherin, Pancreasin, PAPP-A, PD-1, PDGF R beta, PDGF-C, Pepsinogen 1, Pepsinogen 2, Persephin, PGRP-S, Presenilin 1, Procalcitonin, Progranulin, Prolactin, Prostasin, PSA-free, PSA-total, PTH, PYY, RBP4, Ret, ROBO4, Ryk, S100 A8, Serpin A4, sFRP-3, SIGIRR, SLAMF4, SLPI, SOST, SOX2, Spinesin, SR-B2, Syndecan-1, Syndecan-3, TCCR, TFPI, TGF beta R3, THP, Thrombomodulin, Thrombospondin-1, Thrombospondin-2, Thyroglobulin, Tie-1, TIMP-4, TLR1, TLR2, TLR3, TLR4, TNFRSF12A, Transferrin, TREM-1, Troponin I, TSH, TSLP, TYRO3, Ubiquitin+1, uPA, Uromodulin, VEGF-C, VEGFR1, WIF-1, WSX-1, ADAM-12, CA12, Cadherin-11, Cadherin-4, Cathepsin E, CD28, CD34, CD6, CD99, CDO, CILP-1, CXADR, Desmoglein-3, EMR2, Ephrin-A4, Galectin-4, Galectin-8, Galectin-9, Glypican 1, Glypican 2, HAPLN1, Hepsin, ICOS, Jagged 1, Jagged 2, Matrilin-3, MDGA1, MDM2, Nectin-4, NKp30, NKp44, Notch-3, Renin, RGM-A, RGM-B, ROBO2, ROBO3, Semaphorin 6D, Siglec-11, TFPI-2, TREM-2, ULBP-3, Aggrecan, Brevican, CD109, CRTAC1, Cystatin E/M, Cystatin SN, Desmocollin-3, Desmoglein-1, Desmoglein-2, DSPG3, Complement MASP3, LAIR1, Meprin alpha, Nectin-1, Nectin-3, Nidogen-2, Periostin, Semaphorin 6B, Semaphorin 7A, SP-D, Syndecan-4, ADAM-23, TRKC, CDNF, Gas 6, GPR56, GPVI, AmiGO, Amnionless, CD83, CLEC-1, CREG, PAPP-A2, Plexin B3, Plexin D1, ProGRP, Reg1B, LAP/TGF beta 1, ADAM-8, CEA Variant, CEACAM-5 Variant, Galectin-2, ULBP-1, Common beta Chain, Ephrin-B3, FOLR2, Prolactin R, IL-17RA, IL-17RB, IL-23R, IL-32 alpha, LRIG3, Thrombospondin-5, Cathepsin B / Pro-Cathepsin B, Eotaxin-1 / CCL11, Fas Ligand / TNFSF6, IL-8 / CXCL8, MIP-1 alpha / CCL3, RANTES / CCL5, CTLA-4 / CD152, BLC / CXCL13, aFGF / FGF-1, IL-33 / IL-1F11, NCAM-1 / CD56, TNF R1 / TNFRSF1A, IL-1 beta / IL-1F2, CD40 Ligand / TNFSF5, MIG / CXCL9, MIP-1 beta / CCL4, ADP-Ribosyl Cyclase 2 / BST-1 / CD157, Angiopoietin-like protein 3, Angiopoietin-like protein 4, BLAME / SLAMF8, CD27 / TNFRSF7, CD58 / LFA-3, CD84 / SLAMF5, N-Cadherin / Cadherin-2, VE-Cadherin / CDH5, CHL-1 / L1CAM-2, CK beta 8-1 / CCL23, Coagulation Factor VII / F7, Dopa Decarboxylase / DDC, FOLH1 / PSMA, Follistatin-like 1 / FSTL1, Granulysin / NKG5 / LAG-2, IL-10R alpha, IL-10R beta, IL-13R alpha 2, IL-15R alpha, IL-18BPa, IL-38 / IL-1F10, IL-1R-1 / IL-1RT-1 / IL-1R alpha / CD121a / p80, IL-1R-2 / IL-1RT-2 / IL-1R beta / CD121b, IL-20R alpha, IL-21R, IL-22R alpha 1, IL-27R alpha, IL-2R alpha, IL-31R alpha, IL-36 beta / IL-1F8, IL-4R alpha, IL-5R alpha, IL-6R, Insulin R / CD220, Langerin / CD207, Neprilysin-2 / MMEL1, RELT / TNFRSF19L, Siglec-10 / Siglec-G, Siglec-7 / CD328, Siglec-9 / CD329, Tie-2 / TEK, BAFF R / TNFRSF13C, TRANCE / TNFSF11, SCF / KITLG, Activin R2B / ACVR2B, Adipsin / Complement Factor D, Angiotensinogen / Serpin A8, Arylsulfatase A / ARSA, CD86 / B7-2, B7-H3 / CD276, BMPR-IA / ALK-3, BOC / Brother of CDO, PECAM-1 / CD31, CD320 / TCblR / 8D6A, Contactin-2 / TAG1, Coagulation Factor XIV / Protein C / F14, Carboxypeptidase A2 / CPA2, BRAK / CXCL14, DPPII / QPP / DPP7, GDF-8 / Myostatin, ICAM-2 / CD102, IL-13R alpha 1, IL-1R-4 / ST2 / IL-33R, IL-36 gamma / IL-1F9, JAM-C / CD323, LAMP1 / CD107a, LIGHT / TNFSF14, MCP-2 / CCL8, MCP-3 / MARC / CCL7, Meprin beta / MEP1B, CD155 / PVR, Siglec-5 / CD170 / Siglec-F, TIM-3 / KIM-3, TNF R2 / TNFRSF1B, 4-1BB Ligand / TNFSF9, WISP-1 / CCN4, IL-1 alpha / IL-1F1, IP-10 / CXCL10, TWEAK / TNFSF12, ALCAM / CD166, CD40 / TNFRSF5, Endoglin / CD105, TROP1 / EpCAM, ErbB4 / HER4, FCAR / CD89, GITR / TNFRSF18, IL-7R alpha / CD127, JAM-A / CD321 / F11R, Lymphotoxin beta R / TNF RIII / TNFRSF3, Serpin F1 / PEDF, SLAM / CD150, GASP-1 / WFIKKNRP, IL-36Ra / IL-1F5, IL-1ra / IL-1RN / IL-1 F3 / IRAP, MCP-1 / CCL2, CXCL1 / GRO alpha / KC / CINC-1, FGF-7 / KGF, HGFR / MET, TWEAK R / TNFRSF12, Fas / TNFRSF6 / Apo-1, MIP-3 alpha / CCL20, Pentraxin-3 / TSG-14, 2B4 / CD244, 6Ckine / CCL21, ACE / CD143, Activin R1B / ALK-4, Adiponectin / ACRP30, ALK-6 / BMPR-IB, Aminopeptidase LRAP / ERAP2, Aminopeptidase P2 / XPNPEP2, B7-H1 / CD274 / PD-L1, B7-H2 / ICOS Ligand, BAMBI / NMA, Betacellulin / BTC, Cathepsin V / Cathepsin L2, MEC / CCL28, CD147 / EMMPRIN, CD229 / SLAMF3, CD26 / DPPIV, CD300c / CLM-6, CD30 / TNFRSF8, CD48 / SLAMF2, CD80 / B7-1, CHST1 / GST-1, CHST4 / GST-3, CLEC10A / CD301, Calsyntenin-1 / Alcadein-alpha, CMG-2 / ANTXR2, Coagulation Factor III / Tissue Factor / Thromboplastin / F3, CRP / C-Reactive Protein, Cardiotrophin-1 / CT-1, CTACK / CCL27, DcR3 / TNFRSF6B, Dectin-1 / CLEC7A, DNAM-1 / CD226, DR3 / TNFRSF25, DR6 / TNFRSF21, EG-VEGF / PK1, ENA-78 / CXCL5, Endoglycan / PODXL2, Eotaxin-2 / MPIF-2 / CCL24, Eotaxin-3 / CCL26, Fc gamma RIIB/C / CD32B/C, FCRL1 / FcRH1, FCRL2 / FcRH2, Flt-3 / Flk-2, Fractalkine / CX3CL1, GASP-2 / WFIKKN, GCP-2 / CXCL6, GCSF R / CD114, GITR Ligand / TNFSF18, GOLM1 / GP73, HCC-1 / CCL14, HCC-4 / CCL16, HVEM / TNFRSF14 / CD270, I-309 / TCA-3 / CCL1, ICAM-1 / CD54, ICAM-3 / CD50, IL-17E / IL-25, IL-18R beta / IL-1R-7 / AcPL / IL-18RAcP, IL-36 alpha / IL-1F6, IL-37 / IL-1F7, IL-36 gamma / IL-1RP2 / IL-1F9, IL-1R-3 / IL-1RAcP, IL-18 R alpha / IL-1R-5, IL-1Rrp2 / IL-1R-6, IL-28A / IFN lambda 2, IL-29 / IFN-lambda 1, IL-2R beta / CD122, ILT-2 / LIR-1 / CD85j, I-TAC / CXCL11, JAM-B / VE-JAM / CD322, KIRREL3 / NEPH2, Cytokeratin 8 / KRT8, Lipocalin-2 / NGAL, L-Selectin / CD62L, Lymphotactin / XCL1, Matriptase / ST14, MCP-4 / CCL13, MDC / CCL22, MEPE / OF45, MIP-1 delta / CCL15, MIP-3 beta / CCL19, MIS RII / AMHR2, MPIF-1 / CCL23, MSP Receptor / Ron, NAP-2 / PPBP / CXCL7, NGFR / TNFRSF16, Nogo Receptor / Reticulon-4 Receptor, NOV / CCN3, NRG1-beta 1 / HRG1-beta 1, Neurexin-3 beta / NRXN3, Olfactomedin-2 / Noelin-2, Osteoprotegerin / TNFRSF11B, Osteopontin / SPP1, Oncostatin M R beta / IL-31 R beta, Osteoactivin / GPNMB, OX40 / TNFRSF4, OX40 Ligand / TNFSF4, p27/Kip1 / CDKN1B, PARC / CCL18, Platelet Factor 4 / CXCL4, Pref-1 / DLK-1 / FA1, RANK / TNFRSF11A, SCF R / CD117 / c-kit, SDF-1 alpha / CXCL12 alpha, SDF-1 beta / CXCL12 beta, gp130 / sgp130, Sonic Hedgehog N-Terminal / Shh-N, Siglec-5 / CD170, SREC-I / SCARF1, SREC-II / SCARF2, ADAM-17 / TACE, TACI / TNFRSF13B, TARC / CCL17, TECK / CCL25, Testican 2 / SPOCK2, TFF3 / Trefoil Factor 3, TIM-1 / KIM-1, TNF beta / TNFSF1 / LT-alpha, BCMA / TNFRSF17, 4-1BB / TNFRSF9 / CD137, Thrombopoietin / TPO, Tripeptidyl-peptidase I / TPP1, TRACP / PAP / ACP5, TRAIL / TNFSF10, TRAIL R1 / TNFRSF10A / DR4, TRAIL R2 / TNFRSF10B / DR5, TRAIL R3 / TNFRSF10C, TRAIL R4 / TNFRSF10D, TROY / TNFRSF19, PD-ECGF / Thymidine Phosphorylase, ULBP-2 / ALCAN-alpha / N2DL2, VCAM-1 / CD106, TCK-1 / CXCL7, BAFF / TNFSF13B, Thrombospondin 5 / COMP, IL-2R gamma / Common gamma Chain, IL-22R alpha 2, LAMP / LSAMP, IL-1F10 / IL-1HY2 / FIL1 theta

-20°C

640

Sandwich-based

Quantitative

Human Cytokine Antibody Array Q640 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

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